cond1rep1_S1_L002_R2_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond1rep1_S1_L002_R2_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1776607
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGACAGACTGCAGCCATTCGCTCACTGATGAGGAGTAGCAGTGTTGGACGGCTCCCAAGGCGGATGTGGTACAGT	3468	0.19520355374036014	Search with Blastn,more detail First hit on 16: Mus musculus peripheral myelin protein 22 (Pmp22), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
CACATGACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAA	3127	0.1760096633639291	Search with Blastn,more detail First hit on 14: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
CCAGTAAAGACTTAGTGGGGAACTGCAAAAGCCACATGACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAG	3000	0.16886120565775098	Search with Blastn,more detail First hit on 44: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG	2793	0.15720978246736617	Illumina RNA PCR Primer (100% over 50bp)
GGGGAACTGCAAAAGCCACATGACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGA	2788	0.15692834712460324	Search with Blastn,more detail First hit on 45: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
ACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAGATCGTC	2504	0.1409428196556695	No hit
GGAGAAATATACAGAATGTAGAAAAGTCCTAACAGTCCACTGTGCCCGGGGGTGTAGCCACTTCCTTTCCTTCCC	2453	0.13807217915948772	Search with Blastn,more detail First hit on 16: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
ACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAAAAAATA	2427	0.13660871537712055	Search with Blastn,more detail First hit on 14: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
CGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCG	2264	0.1274339232030494	Search with Blastn,more detail First hit on +100: Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
GGAGTCTTAGCATCAGAAGGACACCGTATATACATCTACAGTTGGTGGCCAATACAAGTCATCGCCAGACAGTCC	2090	0.11763997327489985	Search with Blastn,more detail First hit on 76: Mus musculus peripheral myelin protein 22 (Pmp22), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
GTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAGATCGTCGGACTGTAGAACTCTG	1997	0.11240527589950956	Illumina DpnII expression Sequencing Primer (100% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGCGA	1275	13.08542	57
CGAAA	5990	11.674528	47
AACGA	6705	10.111941	45
GACGT	4935	10.070264	6
AAACG	7035	10.041276	44
TACCG	2410	10.015943	6
CGGTC	8300	9.280719	15
CCGCG	1890	9.203101	56
CGTCA	8015	9.00284	2
ACGAA	7975	8.94675	46
TTCGC	3020	8.93319	17
GGCCG	8330	8.522852	24
CTAAC	6650	8.487414	29
CGTGG	8705	8.441148	27
ACGTC	7325	8.258124	1
CCGTG	9065	8.2234	26
GGAGA	18115	8.014233	1
GCCCG	7045	8.011542	44
CCTCG	9055	7.997268	12
ATTCG	3425	7.9804997	16

Bad tiles

No bad tiles

Summary