FastQC Report![[OK]](data:image/png;base64,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)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | cond1rep1_S1_L004_R2_001.fastq.bz2 |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 1788497 |
| Sequences flagged as poor quality | 0 |
| Sequence length | 75 |
| %GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
![[WARN]](data:image/png;base64,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)
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GGACAGACTGCAGCCATTCGCTCACTGATGAGGAGTAGCAGTGTTGGACGGCTCCCAAGGCGGATGTGGTACAGT | 3371 | 0.1884822842867503 | Search with Blastn,more detail First hit on 16: Mus musculus peripheral myelin protein 22 (Pmp22), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
| CACATGACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAA | 3150 | 0.17612554004843173 | Search with Blastn,more detail First hit on 14: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
| GGGGAACTGCAAAAGCCACATGACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGA | 2891 | 0.16164410675556068 | Search with Blastn,more detail First hit on 45: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
| CCAGTAAAGACTTAGTGGGGAACTGCAAAAGCCACATGACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAG | 2822 | 0.15778611873545217 | Search with Blastn,more detail First hit on 44: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
| GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG | 2636 | 0.1473863249421162 | Illumina RNA PCR Primer (100% over 50bp) |
| ACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAGATCGTC | 2603 | 0.1455412002368469 | No hit |
| ACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAAAAAATA | 2493 | 0.13939078455261597 | Search with Blastn,more detail First hit on 14: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
| GGAGAAATATACAGAATGTAGAAAAGTCCTAACAGTCCACTGTGCCCGGGGGTGTAGCCACTTCCTTTCCTTCCC | 2489 | 0.1391671330731894 | Search with Blastn,more detail First hit on 16: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
| CGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCG | 2327 | 0.1301092481564129 | Search with Blastn,more detail First hit on +100: Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
| GGAGTCTTAGCATCAGAAGGACACCGTATATACATCTACAGTTGGTGGCCAATACAAGTCATCGCCAGACAGTCC | 2122 | 0.11864710983580068 | Search with Blastn,more detail First hit on 76: Mus musculus peripheral myelin protein 22 (Pmp22), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
| GTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAGATCGTCGGACTGTAGAACTCTG | 2067 | 0.1155719019936852 | Illumina DpnII expression Sequencing Primer (100% over 23bp) |
![[FAIL]](data:image/png;base64,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)
Adapter Content
Kmer Content
| Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| CGAAA | 5665 | 0.0 | 11.847118 | 47 |
| TTCGC | 3050 | 0.0 | 10.823102 | 17 |
| CGCGA | 1340 | 0.0 | 10.33038 | 57 |
| AAACG | 6865 | 0.0 | 9.980081 | 44 |
| ACGAC | 3440 | 0.0 | 9.802157 | 25 |
| AACGA | 6820 | 0.0 | 9.629788 | 45 |
| GACGT | 5075 | 0.0 | 9.373159 | 6 |
| CGACT | 3350 | 0.0 | 9.112181 | 26 |
| ACGAA | 7670 | 0.0 | 9.0259495 | 46 |
| CGTCA | 7825 | 0.0 | 8.988549 | 2 |
| CGGTC | 8395 | 0.0 | 8.963629 | 15 |
| ATTCG | 3610 | 0.0 | 8.947524 | 16 |
| TACCG | 2825 | 0.0 | 8.796235 | 6 |
| GGCCG | 8120 | 0.0 | 8.654972 | 24 |
| ACGTC | 7335 | 0.0 | 8.4281225 | 1 |
| GGAGA | 18150 | 0.0 | 8.378163 | 1 |
| CGTGG | 8520 | 0.0 | 8.373849 | 27 |
| CTAAC | 6625 | 0.0 | 8.30452 | 29 |
| TCGTA | 3665 | 0.0 | 8.140314 | 51 |
| CCGTG | 8945 | 0.0 | 8.134711 | 26 |
Bad tiles
No bad tiles