cond2rep1_S4_L004_R2_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond2rep1_S4_L004_R2_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1964605
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG	5619	0.28601169191771375	Illumina RNA PCR Primer (100% over 50bp)
GGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC	3018	0.1536186663476882	Search with Blastn,more detail First hit on +100: TPA_exp: Mus musculus ribosomal DNA, complete repeating unit Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
AGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAA	2823	0.14369300699122725	Search with Blastn,more detail First hit on +100: Rhinebothroides cf. freitasi LRP3929 28S ribosomal RNA gene, partial sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
TGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCA	2502	0.1273538446659761	Search with Blastn,more detail First hit on +100: Homo sapiens clone LA13_165F6 sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTAT	3400	10.653056	47
CCGTA	3870	9.723084	46
TACCG	2500	8.378696	6
GTCGC	5125	7.895634	42
ATACG	2975	7.5161676	36
GGAAA	19095	7.4625525	1
ATCGA	4400	7.2612276	60
GGAGA	19910	6.96075	1
CGCAA	3275	6.719288	32
AACGC	4655	6.633664	17
TAGCG	3550	6.3989034	26
CAACG	4935	6.257286	16
CGATT	4285	6.0477285	61
ACGCT	5520	6.0441003	18
CGCTT	5480	5.893913	19
GTACG	2900	5.876201	62
GTATC	7150	5.860422	48
GGAAT	13670	5.770948	1
TACGA	3650	5.7372265	37
ACGTA	3395	5.646434	60

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