cond2rep2_S5_L001_R1_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond2rep2_S5_L001_R1_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1841075
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC	5098	0.2769034395665576	Search with Blastn,more detail First hit on +100: Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	3191	0.17332265116847492	TruSeq Adapter, Index 5 (100% over 63bp)
TGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTC	2557	0.13888624852328124	Search with Blastn,more detail First hit on +100: PREDICTED: Cavia porcellus collagen alpha-1(I) chain-like (LOC100717127), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTAT	3490	8.239195	45
CGTCC	6135	8.101	22
TCGTA	3800	7.660471	44
GGTCG	4030	7.0470953	14
CTAGG	7135	7.015366	50
CGAAC	4280	6.5525227	50
GCGAA	4205	6.5005474	37
AATCG	4230	6.4621277	47
TCGGT	4285	6.4620304	12
CCTAG	8615	6.387069	49
GTCGT	4515	6.290098	15
GTCCG	4255	6.2572985	18
TATAT	11010	6.193719	1
GCCGT	5115	6.176886	50
CGGTC	4670	6.157343	13
GACGT	7865	6.0482717	20
CCCTA	9805	5.9739494	48
TCGAA	5055	5.758613	49
ATATT	11920	5.7182345	2
TCGTC	5755	5.613339	16

No bad tiles